TY - JOUR ID - 255141 TI - Cytotoxic effects of Anacyclus pyrethrum, Terminalia chebula, and Brassica nigra ethanol extracts on KB cancer cell line JO - International Journal of New Chemistry JA - IJNC LA - en SN - 2645-7237 AU - Fattahi, Shirin AU - Baradaran, Behzad AU - Aghbali, Amir Ala AU - Emamverdizadeh, Parya AU - Chaghakaboodi, Katayon AD - Department of Oral and Maxillofacial Pathology,Faculty of Dentistry,Tabriz University of Medical Sciences,Tabriz,Iran. AD - Immunology Research Center,Tabriz University of Medical Sciences,Tabriz,Iran AD - Department of Oral and Maxillofacial Pathology,Faculty of Dentistry,Tabriz University of Medical Sciences,Tabriz,Iran AD - Department of Oral and Maxillofacial Pathology,Faculty of Dentistry,Kermanshah University of Medical Sciences,Kermanshah,Iran Y1 - 2023 PY - 2023 VL - 10 IS - 1 SP - 35 EP - 42 KW - Anacyclus pyrethrum/pellitory KW - Terminalia chebula KW - Brassica nigra KW - Oral Squamous Cell Carcinoma KW - Apoptosis DO - 10.22034/IJNC.2023.1.7 N2 - The prevalence of oral squamous cell carcinoma (OSCC) has increased in the last 2 decades. For the treatment of this disease, various herbal anticancer agents have been introduced. In this study, for the first time, we analyzed the apoptotic and cytotoxic effects of herbal plants, including Anacyclus pyrethrum, Terminalia chebula, and Brassica nigra, in KB cancer cell lines. For analyzing the cytotoxic effects of ethanol extracts, MTT reduction assay was performed in the KB cells. Moreover, the apoptosis-inducing effect of plants on KB cells was assessed using TUNEL and DNA fragmentation assays. The results showed that Anacyclus pyrethrum and Terminalia chebula extracts significantly inhibited cell viability. TUNEL and DNA fragmentation assays showed that the main mechanism of cell death was apoptosis induction by Anacyclus pyrethrum and Terminalia chebula extracts. Our results suggest that these ethanol extracts may contain bioactive constituents, which can be helpful in OSCC treatment. UR - https://www.ijnc.ir/article_255141.html L1 - https://www.ijnc.ir/article_255141_960ebc80f6e91a2aecaa4b17f7196538.pdf ER -