TY - JOUR ID - 40549 TI - Isolation, Purification and Identification of β-carotene from Azolla Pinnata R. Br. as a New Carotenoid Wealthy Source JO - International Journal of New Chemistry JA - IJNC LA - en SN - 2645-7237 AU - Ghahari, Sajjad AU - Ghahari, Somayeh AU - Nematzadeh, Ghorban Ali AD - Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Iran Y1 - 2021 PY - 2021 VL - 8 IS - 3 SP - 265 EP - 276 KW - Azolla KW - β-Carotene KW - Column chromatography KW - HPLC KW - Natural product DO - 10.22034/ijnc.2020.122084.1098 N2 - Azolla is a wealthy source of β-carotene. The purpose of this study was to isolate, purify and identify the β-carotene in Azolla Pinnata R. Br. plant. In this order, dry and fresh of A. Pinnata R. Br. were used and their β-carotene was extracted using the Acetone/Hexane solvent system. For the purification of β-carotene extracted, the column chromatography was used. In the following, an HPLC technique optimized for β-carotene designation and compared with a spectrophotometric standard method. The most appropriate sample conditions were: extraction with Hexane/Acetone 60:40 (v/v) and MeOH/THF/Water 67:27:6 (v/v) as mobile phase. The results indicated that the fresh sample has almost two times higher β-carotene comparing to the dried sample. So due to the importance of β-carotene as an antioxidant in one hand and the free of cost of mass production of A. Pinnata on another hand, the application of the water fern can be feasible for commercially β-carotene purification. UR - https://www.ijnc.ir/article_40549.html L1 - https://www.ijnc.ir/article_40549_5dccf2b728796ec1fe35a934c3044bec.pdf ER -