In this report, a simple and eco-friendly biosynthesis of copper nanoparticles using Pomegranate leaves extracts as the reducing agent from CuNPs had been investigated. The formation of copper nanoparticles was characterized by UV-Vis spectrum, Fourier Transform Infrared Spectroscopy (FT-IR), and Scanning Electron Microscopic (SEM) analysis. The UV-Vis spectra results show a strong resonance centered on the surface of silver nanoparticles (CuNPs) at 280 nm. The Fourier Transformation Infrared Spectroscopy spectral study demonstrates Pomegranate leaves extract acted as the reducing agent. The scanning electron microscopic (SEM) analysis shows nanoparticles with an average particle size ranges about 19.82-38.15 nm. The structural characterization was carried out using XRD consistent with (111), (200), (311), and (400) reflections of the face-centered cubic (fcc) phase of the CuNPs .The biologically synthesized copper nanoparticles were found to be effective in controlling the growth of human pathogens viz., Salmonella. This route is rapid, simple, without any hazardous chemicals as reducing or stabilizing agents, and economical to synthesize CuNPs.