Herein we report the biogenic synthesis of Pd/CuFe2O4 magnetic nanocatalyst using Lantana camara flowers for the Sonogashira cross-coupling reaction. The as-prepared magnetic nanoparticles have been extensively characterized by powder X-ray diffraction (PXRD), SEM, EDX, TEM, HRTEM, ICP and BET surface area measurement techniques. The activity of different catalyst such as CuFe2O4, Pd/NiFe2O4 and Pd/Fe3O4 were investigated for Sonogashira cross-coupling reaction and it was observed that Pd/CuFe2O4 showed superior catalytic activity compared to the other catalyst systems. The use of non-toxic commercially available solvents, mild reaction conditions, easy preparation of the catalyst, high yield of the products and less reaction time are the advantages of the present protocol. The catalyst showed higher selectivity towards coupling of aryl halides with alkynes resulting in moderate to excellent isolated yields. The catalyst was easily separated with the help of an external magnet and reused upto 5th cycle without any significant loss in the catalytic activity and product yield.