A selected solid complex of the Schiff base ligand derived from Glutaric anhydride with Cu(II) ion was synthesized and characterized by FT-IR, Electronic, ESR Spectral Analyses, Magnetic susceptibility and Molar Conductance Measurements. The disappearance of ν(O-H) hydroxyl band of the phenolic and the lowering shift of the stretching frequency of the ν(CH=N) azomethine band in the ligand after complexation, indicated the coordination through the phenolic oxygen atom (after deprotonation) and azomethine nitrogen atom respectively of the Schiff base ligand. The lower values of molar conductance indicate the non-electrolytic nature of these complex. The ESR spectrum of the Copper complex has octahedral geometry. The Schiff base ligand and its complex further identified by 1H NMR, 13C NMR, SEM, EDX and molecular docking study.The anticancer potential of the Copper (II) complex was determined against A549 lung cancer cells, they exhibited appreciable anticancer activity. The in vitro cytotoxicity of the complex was examined against cancer cell line by MTT assay.The Schiff base Cu (II) complex was tested its cytotoxicity and found that the 50 percentage of activity inhibitory concentration (IC50) value around 91.25 percentage in 7.8 µg/ml.