Comparative study of in-vitro antidiabetic property in the methanol extract of stem bark and leaves of Semecarpus anacardium Linn (Bhalayo) from Nawalparasi district, Nepal

Bhatt, Bishan Datt

doi:10.22034/ijnc.2024.2030765.1397

Comparative study of in-vitro antidiabetic property in the methanol extract of stem bark and leaves of Semecarpus anacardium Linn (Bhalayo) from Nawalparasi district, Nepal

Document Type : Research Paper

Author

Bishan Datt Bhatt

Tri-Chandra Multiple Campus, Tribhuvan University, Nepal

https://doi.org/10.22034/ijnc.2024.2030765.1397

Abstract

Semecarpus anacardium Linn (Bhalayo) has been used traditionally for ages as it can cure and control several incredible diseases including diabetes mellitus. In the present work, an investigation has been carried out to compare the in-vitro antidiabetic activity in the methanol extract of stem bark and leaves of Semecarpus anacardium Linn (Bhalayo) from Nawalparasi district of Nepal. Results from phytochemical screening in methanol (MeOH) extracts of stem bark and leaves of this plant discovered secondary metabolites like alkaloids, flavonoids, polyphenols and terpenoids. This demonstrates antioxidant and antidiabetic property with several other medicinal values of this plant. The in-vitro α-amylase enzyme inhibition activity of MeOH extract of either part of this plant was performed using starch as substrate, α amylase as enzyme, and acarbose as standard. The IC50 values of MeOH extract of stem bark and leaves were observed to be 88.26 ± 1.15 µg/mL and 195.61 ± 1.74 µg/mL, respectively. Nevertheless, the IC50 value of MeOH extract of stem bark, analogous to MeOH extract of leaves, seems fairly close to IC50 value of standard drug acarbose, 28.48 ± 0.07 µg/mL. Since, MeOH extract of stem bark of the plant unveiled its potent antidiabetic activity, stem bark of the plants can be employed for the isolation of active compounds to contrive new and efficacious antidiabetic drugs.

Keywords

diabetes

antidiabetic

phytochemicals

α-amylase

Subjects

Phytochemistry

[1] A.N. Singab, F.S. Youssef and M.L. Ashour, Med. Aromat. Plant. 3(1), 1 (2014).
[2] P.A. Akah, S.U. Uzodinma and C.E. Okola, J. Appl. Pharm. Sci. 01, 99 (2011).
[3] K. Gawli and N. Lakshmidevi, Pharm. Biol. 53, 689 (2015).
[4] P.P. Gupta, J. Haider, R.P. Yadav and U. Pal, J. Phytopharm. 5, 45 (2016).
[5] N. Malviya, S. Jain and S. Malviya, Acta. Pol. Pharm. 67, 113 (2010).
[6] M. Sahu, K.V. Sahoo, V. Alagarsamy and R.B. Rath, Austin J Pharmacol Ther. 4, 1081
(2016).
[7] G.M. Karau, M. Sc. thesis, Kenyatta University, 2014.
[8] T. Cheriet, M. Hanfer, A. Boudjelal, N. Baali, I. Mancini, R. Seghiri, S. Ameddah, A.
Menand, F. Benayache and S. Benayache, Nat. Prod. Res. 31, 2042 (2017).
[9] P. Tayde, J. Mahatma Gandhi Inst. Med. Sci. 24, 75 (2019).
[10] A. Roy and G. Mahalingam, Int. J. Green Pharm. 11, 128 (2017).
[11] M.T. Akhtar, M.S.B.M. Sarib, I.S. Ismail, F. Abas, A. Ismail, N. H. Lajis and K. Shaari,
Molecules. 21, 1026 (2016).
[12] N.E.I. Omari, K. Sayah, S. Fettach, O.E.I. Blidi, A. Bouyahya, M.E.L.A. Faouzi, R. Kama
and M. Barkiyou, Evi-Bas Evid-Based Complementary Altern Med. 2019, DOI:
https://doi.org/10.1155/2019/7384735.
[13] B.D. Bhatt and C. Chhetry, J Nepal Chem Soc. 38, 58 (2018).
[14] S. Arunachalam, S. Sundaramoorthy, A. Rastogi and M. Sathiavelu, Int. J. Green Pharm.
11, 128 (2013).
[15] E. Sucharitha and M. Estari, Bio. Med. 5, 20 (2013).
[16] M.S. Sikarwar, M.B. Patil, C.K. Kokate, S. Sharma and V. Bhat, J. Young Pharm. 1, 330
(2009).

[17] J. Aseervatham, S. Palanivelu and S. Panchanadham, Evid-Based Complementary Altern.
Med., 2011. DOI: https://doi.org/10.1155/2011/142978.
[18] M.A. Indap, R.Y. Ambaye and S.V. Gokhale, Indian J. Physiol. Pharmacol. 27, 83 (1983).
[19] M.A. Ali, M.C. Sulatana, B.M. Rahman, N.A. Khatune and M.I,I. Wahed, J. Pharm. Sci.
Res. 3, 2680 (2012).
[20] B. Panigrahi and M. Vyas, Int. J. Pharma. Bio. Sci. 6, 155 (2015).
[21] N. Kumar, G.B. Bevara, K. Laxmikoteswramma and R. Malla, Asian J. Pharm. Clin. Res.
6, 213 (2013).
[22] K. Bhatia, R. Kataria, A. Singh, Z.H. Safderi and R. Kumar, Indian J. Basic Appl. Med.
Res. 3, 122 (2014).
[23] T. E. Kwape, R.T. R. Majinda and P. Chaturvedi, Cogent Biol. 2(1), (2016). DOI:
https://doi.org/10.1080/23312025.2016.1275403.
[24] K. Jayakumar, B. Muthuraman and S. Rajasekaran, World News Nat. Sciences. 16, 97
(2018).
[25] R. Kusano, S. Ogawa, Y. Matsuo, T. Tanaka, Y. Yazaki and I. Kouno, J. Nat. Prod. 74, 119
(2011).