Analysis of Chemical Composition of Pumpkin Seed Extract (Cucurbita pepo) and its Effect on Hematological Parameters and Growth in Rainbow Trout (Oncorhynchus mykiss)

Vakili, Kaveh; Khajeh Rahimi, Amir Eghbal; Salimi, Behnam; Kakulaki, Shapur; Ghorbanzadeh, Arman

doi:10.22034/ijnc.2024.715478

Analysis of Chemical Composition of Pumpkin Seed Extract (Cucurbita pepo) and its Effect on Hematological Parameters and Growth in Rainbow Trout (Oncorhynchus mykiss)

Document Type : Research Paper

Authors

Kaveh Vakili ¹

Amir Eghbal Khajeh Rahimi ¹

Behnam Salimi ²

Shapur Kakulaki ¹

Arman Ghorbanzadeh ¹

¹ Department of Food Hygiene,Science and Research Branch, Islamic Azad University, Tehran, Iran.

² Department of Aquatic Animal Health and Disease, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.

https://doi.org/10.22034/ijnc.2024.715478

Abstract

The aim of this study was to identify and analyze the chemical composition of pumpkin seed extract (Cucurbita pepo) and to evaluate its effect on hematologic factors and growth indices in rainbow trout (Oncorhynchus mykiss). The extract was prepared and analyzed using standard chemical methods and the fish were divided into four groups (one control and three treatments). Blood factors and growth indices were measured on days 0, 30 and 60. The results showed that treatment with pumpkin seed extract significantly increased hematological parameters such as RBC and PLT as well as improved growth indices. This study shows that pumpkin seed extract contains chemically active compounds that can be effective in improving the health and growth of farmed fish.

Keywords

Rainbow trout

squash

Yersinia ruckeri bacteria

M. Turchini, K. M. Hermon, D. S. Francis, Aquaculture. 491, 391(2018).
Kumar, C. R. Engle, Reviews in Fisheries Science & Aquaculture. 24, 136 (2016).
A. Ansari, A. Guldhe, S. K. Gupta, I. Rawat, F. Bux, Environmental Science and Pollution Research. 28, 43234 (2021).
S. Hossain, B. C. Small, V. Kumar, R. Hardy, Reviews in Aquaculture. 16, 121 (2024).
M. Greiling, C. Schwarz, M. Gierus, M. Rodehutscord, Archives of animal nutrition. 72, 239 (2018).
Dowidar, A. Ahmed, H. Mohamed, Zagazig Veterinary Journal. 48, 199 (2020).
Tian, Y. Bai, H. Tian, X. Zhao, Molecules. 28, 6393 (2023).
A. Triantaphyllopoulos, D. Cartas, H. Miliou, Reviews in Aquaculture. 12(3), 1637 (2020).
Vakili, A. E. Khajehrahimi, B. Salimi, S. Kakoolaki, A. Ghorbanzadeh, Journal of the Selva Andina Research Society. 15, 3 (2024).
Shouhani, A. Hemati Ali, M. Taheri Moghadam, J Ilam Univ Med Sci. 17, 9 (2010).
Ghalebi Hajivand, A. Smaeili, A. Abedian, Iran Sci Fish J. 28, 27 (2019).
G. Tacon, N. Rausin, M. Kadari, P. Cornelis, Aquaculture Research. 21(4), 375 (1990).
Siddiqui, A. Al-Harbi, Aquaculture. 138, 145 (1995).
J. Walker, J. R.Winton, Vet Res. 41(6), 113 (2010).
Adel, C. M. A. Caipang, M. A. O. Dawood, Fish Shellfish Immunol. 71, 230 (2017).
Nawirska-Olszańska, A. Kita, A. Biesiada, A. Sokół-Łętowska, A. Z. Kucharska, Food Chem. 139,155 (2013).
Z. H. Eleiwa, R. O. Bakr, S. A. Mohamed. Int J Pharmacogn Phytochem. 29, 1226 (2014).
H. Rezaei, I. Sourinejad, S. Soltanian, M. Yousefzadi, J Aquat Ecol. 3, 18 (2013).