Cytotoxic effects of Anacyclus pyrethrum, Terminalia chebula, and Brassica nigra ethanol extracts on KB cancer cell line

Fattahi, Shirin; Baradaran, Behzad; Aghbali, Amir Ala; Emamverdizadeh, Parya; Chaghakaboodi, Katayon

doi:10.22034/IJNC.2023.1.7

Cytotoxic effects of Anacyclus pyrethrum, Terminalia chebula, and Brassica nigra ethanol extracts on KB cancer cell line

Document Type : Original

Authors

Shirin Fattahi ¹

Behzad Baradaran ²

Amir Ala Aghbali ¹

Parya Emamverdizadeh ³

Katayon Chaghakaboodi ⁴

¹ Department of Oral and Maxillofacial Pathology,Faculty of Dentistry,Tabriz University of Medical Sciences,Tabriz,Iran.

² Immunology Research Center,Tabriz University of Medical Sciences,Tabriz,Iran

³ Department of Oral and Maxillofacial Pathology,Faculty of Dentistry,Tabriz University of Medical Sciences,Tabriz,Iran

⁴ Department of Oral and Maxillofacial Pathology,Faculty of Dentistry,Kermanshah University of Medical Sciences,Kermanshah,Iran

https://doi.org/10.22034/IJNC.2023.1.7

Abstract

The prevalence of oral squamous cell carcinoma (OSCC) has increased in the last 2 decades. For the treatment of this disease, various herbal anticancer agents have been introduced. In this study, for the first time, we analyzed the apoptotic and cytotoxic effects of herbal plants, including Anacyclus pyrethrum, Terminalia chebula, and Brassica nigra, in KB cancer cell lines. For analyzing the cytotoxic effects of ethanol extracts, MTT reduction assay was performed in the KB cells. Moreover, the apoptosis-inducing effect of plants on KB cells was assessed using TUNEL and DNA fragmentation assays. The results showed that Anacyclus pyrethrum and Terminalia chebula extracts significantly inhibited cell viability. TUNEL and DNA fragmentation assays showed that the main mechanism of cell death was apoptosis induction by Anacyclus pyrethrum and Terminalia chebula extracts. Our results suggest that these ethanol extracts may contain bioactive constituents, which can be helpful in OSCC treatment.

Keywords

Anacyclus pyrethrum/pellitory

Terminalia chebula

Brassica nigra

Oral squamous cell carcinoma

Apoptosis

Subjects

Applied Chemistry

[1]. F Judah, Cancer Spectrum Knowledge Environment, 82:4 (1990)
[2]. E. Ghaibi E; et al., Eurasian Journal of Chemical, Medicinal and Petroleum Research, 1:49
(2022)
[3]. K. Lo Han, Eurasian Journal of Chemical, Medicinal and Petroleum Research, 1:64 (2022)
[4]. N Shahkarami, et al., Eurasian Chemical Communications 4:463 (2022)
[5]. A Manosroi A, et al., Nat Prod Res. 24:1915 (2010)
[6]. HS Lee, Biol Pharm Bull. 28:1639 (2005)
[7]. JH Kim, et al., Phytother Res. 26:39 (2012)
[8]. A Saleem, et al., fruit. J Ethno-pharmocol. 81: 327 (2002)
[9]. TN Sairam, et al., J Nanosci Nano technol. 9:5471 (2009)
[10]. S Kaur, et al., Mutat Res. 419: 169 (1998)
[11]. F Malekzadeh, et al., Int J Antimicrob Agents. 18:85 (2001)
[12]. AG Jagtap, SG Karkera, J Ethno-pharmocol. 68:299 (1999)
[13]. CJ Li, et al., Evid Based Complement Alternat Med. 2012:26197 (2012)
[14]. Y Lu, et al., Hepatobiliary Pancreat Dis Int. 10: 303 (2011)
[15]. H Ma, et al., Phytother Res. 22: 1472 (2008)
[16]. EJ Buenz, et al., Trends in Pharmacological Sciences, 25:494 (2004)
[17]. YZ Cai, et al., Life Sciences, 74:2157 (2004)
[18]. RL Prior, et al., Journal of Agricultural and Food Chemistry, 51:3273 (2003)
[19]. F Rebout, Eurasian Journal of Chemical, Medicinal and Petroleum Research, 1:58 (2022)
[20]. R Liu, et al., China journal of Chinese materia-medica, 38:4181 (2013)
[21]. WJ Craig, Am J Clin Nutr. 70:491S (1999)
[22]. V Kuete, et al., international journal of phytotherapy and phytopharmacology. 21:315 (2014)
[23]. H Asadi, et al., Advanced Pharmaceutical Bulletin, 4:455 (2014)
[24]. A Azadmehr, et al., Cell proliferation, 46:637 (2013)
[25]. S Valiyari, et al., Toxicological & Environmental Chemistry, 95:1208 (2013)
[26]. A. Johnson, Eurasian Journal of Chemical, Medicinal and Petroleum Research, 2: 1 (2023)