International Journal of New Chemistry
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Response Surface Methodology Applied to the Supercritical Carbon Dioxide Extraction of Zingiber officinale Oleoresin

Document Type : Research Paper

Authors

Shahin Ahmadi 1
Sedighe Pardis kian 2
Maryam Moghaddas 2

1 Department of Pharmaceutical Chemistry, Tehran medical sciences, Islamic Azad University, Tehran, Iran.

2 Department of Chemistry, Safadasht Branch, Islamic Azad University, Tehran, Iran

10.22034/ijnc.2025.722671
Abstract
This study explores the extraction of oleoresin from the rhizome of ginger (Zingiber officinale) using supercritical carbon dioxide (SC-CO₂) extraction and Soxhlet extraction techniques. Key operational parameters for SC-CO₂ extraction, including pressure (10–20 MPa), temperature (35–45 °C), and flow rate (10–16 g min⁻¹), were optimized to evaluate their influence on extraction yield, radical scavenging activity, and total phenolic content. A Box–Behnken design was employed for experimental design and analysis. Regression analysis confirmed that the experimental data conformed well to both linear and second-order polynomial models. The SC-CO₂ method achieved a maximum oleoresin yield of 6.47 ± 0.07%, significantly higher than the 3.19 ± 0.22% obtained using Soxhlet extraction. The antioxidant potential of the extracts, determined through 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, revealed values of 50.70 ± 0.52% for SC-CO₂ extraction and 88.50 ± 0.18% for Soxhlet extraction. The total phenolic content, quantified via the Folin–Ciocalteu method, was 103.24 ± 1.58% for SC-CO₂ extracts under optimal conditions, compared to 31.10 ± 0.28% for Soxhlet extracts.

Keywords

Zingiber officinale
Oleoresin
Supercritical carbon dioxide
Response surface optimization
Antioxidant activity
Total phenolic content

Subjects

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International Journal of New Chemistry
Volume 12, Issue 5
Spring 2025
Pages 972-994

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